Fluorescent Proteins/lacZ System 条件を付加する。<br>学術機関の学術研究に限る。営利機関の利用希望者は事前に利用条件等につき寄託者と合意し、提供承諾を得ること。利用者はApproval form (form V)を用いて、事前にKikGRの開発者の許可を得る(連絡先:Laboratory for Cell Function Dynamics, RIKEN CBS:mta-cfds@ml.riken.jp)。 pCAGGS expression vector の開発者の大阪大学・宮崎純一博士 (FAX: +81-6-6879-3829、e-mail:jimiyaza@nutri.med.osaka-u.ac.jp) に指定の MTA を提出する。研究成果の公表にあたって謝辞の表明を必要とする。研究成果の公表にあたって寄託者の指定する文献を引用する。Tomura M et.al, Sci. Rep. e6030 (2014). 利用者は当該発表物1部を寄託者に送付する。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。 M1 [(C57BL/6 x 129)F1] 古関 明彦 The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the kikGR using Approval form (form V) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: mta-cfds@ml.riken.jp). The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a MTA regarding the usage of the CAG promoter specified by the DEVELOPER, Dr. Junichi Miyazaki. Osaka University (FAX:+81-6-6879-3829, e-mail: jimiyaza@nutri.med.osaka-u.ac.jp). In publishing the research results to be obtained by the use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Tomura M et.al, Sci. Rep. e6030 (2014). The RECIPIENT must send the DEPOSITOR a reprint of the RECIPIENT's publication. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. Developed by Michio Tomura, Kyoto University Graduate School of Medicine. M1 ES cells were used to generate the mutant mice. B6.Cg-Gt(ROSA)26Sor<tm1(CAG-kikGR)Kgwa> B6.Cg-Gt(ROSA)26Sor<tm1(CAG-kikGR)Kgwa> CAG-lox-STOP-lox-kikGR ROSA26 knock-in mice. C57BL/6 background (RBRC09254), BALB/c background (RBRC09255). B6-ROSA/kikGR(floxed), B6-ROSA/kikGRfloxed KI B6-ROSA/kikGR(floxed), B6-ROSA/kikGRfloxed KI RBRC09254 C (3-6 months) true Heterozygote x Wild-type [C57BL/6JJcl] Heterozygote x Wild-type [C57BL/6JJcl] Haruhiko KOSEKI 京都大学大学院医学研究科・戸村道夫先生。M1 ES細胞を用いて作出。 ROSA26 locusにCAG-lox-stop-lox-KikGRをノックインしたマウス。Cre recombinaseにより、green-to-red photoconvertible fluorescence protein, kikGRを発現する。C57BL/6背景 (RBRC09254) 、BALB/c背景 (RBRC09255) 。 <a href='https://brc.riken.jp/mus/pcr09254'>Genotyping protocol -PCR-</a> C(3〜6か月) Cre/loxP system Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>CAGGS MTA (<A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/CAGGS_MTA.docx">English</A>)</li><li>Approval form (form V) from the developer (<A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/KikGR_form_V.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), phage P1 loxP sites, Stop, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, Favia favus Kikume-Green Red cDNA, chicken poly(A) sequence, mouse Gt(ROSA)26Sor genomic region <A HREF="https://mus.brc.riken.jp/ja/mouse_of_month/feb_2017_mm" target="_blank">Mouse of the Month Feb 2017</A>